AHS Serotype diagnosis: Second Step
Different AHSV serotypes were stablished based on neutralization techniques, seroneutralization and virusneutralization test, because neutralizing antibodies (anti-VP2 mainly) are serotype specific.
In the last years, it was shown that seg-2 (VP2) of genome is highly divergent among the nine serotypes of AHSV. A logical conclusion was to develop serotype-specific molecular tests. The development of type-specific RT-PCR targeting AHSV Seg-2 for identification and differentiation of AHSV genotypes, provides a rapid typing method for AHSV in tissue samples, blood or supernatant from virus isolation.
Gel-based (Maan et al., 2011; Sailleau et al., 2000) and real-time RT-PCR methods (Koekemoer, 2008; Bachanek-Bankowska, et al., 2014 y EURL sin publicar) are available for this purpose.
| Serotype identification methods |
|---|
| AFRICAN HORSE SICKNESS |
| Detection of ab against Orbivirus by SNT |
| rRT-PCR for AHSV typing (EURL) |